Zymolyase produced by Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It hydrolyzes linear glucose polymers at beta-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.
Appearance: Light brown, lyophilized powder
Storage: 4 °C
Activity: 22,000 U/g
Essential enzyme: Beta-1,3-glucan laminaripentaohydrolase
Other enzyme components: Protease, Mannanase, and trace amount of Amylase, Xylanase, Phosphatase
Stable pH: 5-10
Optimum working pH and temperature condition:
For lysis of viable yeast cells: pH 7.5, 35°C.
For hydrolysis of yeast glucan: pH 6.5, 45°C.
Heat stability:
At 30°C, 70% of lytic activity is lost after storage for 3 months
At 60°C, lytic activity is lost on incubation for 5 minutes
Activator: Cystein, 2-mercaptoethanol, Dithiothreitol etc.
*Unit definition: One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm (A800) of the reaction mixture under the following assay condition:
Enzyme solution: 1 ml (0.05-0.1 mg/ml for Zymolyase-20,000 U/g)
Brewer's yeast cell suspension: 3 ml (2 mg/ml)
M/15 Phosphate buffer, pH 7.5: 5 ml
Distilled water: 1 ml
Assay procedures: After incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer's yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer's yeast.
For research use and further manufacturing. Not intended for human or animal therapeutic or diagnostic procedures.